Quality of standard ELISA kit depends on which two points - Database & Sql Blog Articles

Why does standard fetal bovine serum require more attention? Its quality largely depends on two key factors, which are outlined below.

Let’s take a closer look at the details.

Detailed Introduction:

First, using standard fetal bovine serum requires careful handling. It is primarily used in cell culture, where maintaining sterility is crucial. Sometimes, inactivation treatment is necessary. However, opinions vary on whether this step is beneficial. Experts suggest that some ELISA kits may contain residual bacteria from production, which can contaminate cultures. Inactivating fetal bovine serum can also destroy nutrients, potentially harming experiments. If you choose high-quality products from reliable manufacturers, inactivated serum can still be effective.

Once inactivated, the serum might have minimal or no effect on cell growth, and excessive heat treatment could reduce serum quality, slowing down cell proliferation. Heat-treated serum often forms more precipitates, which appear as "small black spots" under an inverted microscope. These can be mistaken for contamination, especially when kept at 37°C, leading researchers to wrongly assume microbial growth.

Second, standard fetal bovine serum has specific characteristics. It is derived from blood collected within two hours of a calf's birth. The serum is a light yellow, clear liquid, making it ideal for various applications.

Third, its uses include biochemical research, particularly in cell and tissue culture. Compared to newborn bovine serum, it contains higher levels of proteins and growth factors, which support better cell growth and reproduction.

Fourth, the quality of ELISA kits relies on two main aspects: the source material and the extraction process. To ensure high-quality fetal bovine serum, the following standards must be met:

1. Source Material: The calves should be healthy and free from disease. The serum must be extracted within the specified time frame and undergo strict quality testing.

2. Extraction Process: The procedure must follow precise protocols, ensuring sterility through filtration, absence of viral inhibitors, and freedom from bacterial, fungal, mycoplasma, and viral contamination. Some countries also require no bacteriophage contamination.

The serum should also support optimal cell growth and come from regions or herds with documented disease-free status, along with proper monitoring systems.

Hengyuan Immunology’s third-party testing center offers premium fetal bovine serum, representing top brands like Gibco, Sigma, and Hyclone. Contact us today for expert guidance and high-quality solutions!