Quality of standard ELISA kit depends on which two points - Database & Sql Blog Articles

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Why does standard fetal bovine serum require more care? Its quality depends on two key factors, which are explained below.

Let's dive into the details.

Detailed Introduction:

First, using standard fetal bovine serum requires extra attention. It is primarily used in cell culture, and the most critical factor during this process is to ensure that the culture remains uncontaminated. In some cases, inactivation treatment may be necessary. However, opinions vary regarding whether or not to inactivate fetal bovine serum.

Experts suggest that some ELISA kits may contain bacterial residues from the production process, which can lead to contamination of the culture samples. When inactivating fetal bovine serum, it might kill some nutrients, which could actually harm the experiment. If you choose products from reputable manufacturers, inactivated fetal bovine serum can still perform well.

After inactivation, the serum may have only a slight effect on cell growth, or even no effect at all. High-temperature treatments can also reduce the quality of the serum, leading to slower cell growth. Heat-treated serum tends to form more precipitates, which appear as "small black spots" under an inverted microscope. These can be mistaken for microbial contamination. When kept at 37°C, these precipitates increase, making researchers think they are microbial growth.

Second, standard fetal bovine serum has specific characteristics. It refers to serum prepared from blood taken within two hours of a calf’s birth. The serum is a light yellow, clear, and transparent liquid.

Third, its use is mainly in biochemical research, especially for cell and tissue culture. Compared to newborn bovine serum, fetal bovine serum is more complex, with higher protein content and various growth factors, which are beneficial for cell growth and reproduction.

Fourth, the quality of the ELISA kit depends on two main aspects: the source of the material and the process of collection.

The fetal bovine serum used must come from healthy, disease-free calves. The extraction process should strictly follow procedures, and the serum must undergo rigorous quality checks. Additional requirements include:

• a. Filtering to ensure sterility;
• b. Proving that the serum does not contain inhibitors of vaccine viruses;
• c. Being free from bacteria, mold, mycoplasma, and viruses; some countries also require no bacteriophage contamination;
• d. Providing good support for cell reproduction;
• e. Sourced from a herd or country with documented absence of bovine spongiform encephalopathy, and with proper monitoring systems in place.

Hengyuan Immunology’s third-party testing center offers high-quality fetal bovine serum, representing top brands like Gibco, Sigma, and Hyclone. Feel free to reach out for more information!